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Chicken Embryo Perfused with BriteVu Contrast Media

May 27, 2015/ Comments Off / in Birds
Chicken Embryo Perfused with BriteVu Contrast Media

8 day chicken embryo, vascular perfusion with BriteVu.  10µm CT scan.  BriteVu has been successfully used on many embroys further demonstrating its versatility.  Not only can BriteVu be used on adult developed vascular systems, it can also be used to perfuse developing blood vessels.  This can also prove effective when studying angiogenesis that occurs with growth, organ regeneration, cancer and more.  Courtesy of Kirsten Cable & Norman Hu, Department of Pediatrics, University of Utah

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What size needle can properly prepared BriteVu pass through?

May 21, 2015/ Comments Off / in FAQ

Liquid BriteVu can easily pass through a 27 gauge needle.  If cooled below 37o C (98.6o F) or not properly mixed, liquid BriteVu will being to harden or form clumps.  If this occurs, first make sure the total water volume is adequate (adjust for evaporation if needed) and heat the solution to 70-80o C (140-158o F) for at least 1 minute.  Even at low temperatures (40oC) BriteVu will pass through a 30 g needle.  However, higher temperatures (60-80oC) will ease…

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Can BriteVu be used in animals other than mice and rats?

Apr 30, 2015/ Comments Off / in FAQ

Yes, BriteVu can conceivably be used in any animal as long it can be perfused into the vasculature, body cavity or other location. To date, BriteVu has been successfully used in a variety of mammals, birds and reptiles plus human cadavers. While most studies are vascular based, BriteVu has been used to define respiratory spaces (sinuses, bronchial tree, air sacs, etc), intra-articular (joint) margins, intestinal lumens and more  We have plans of using BriteVu in amphibians, fish and invertebrates- stay…

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How is BriteVu dosed for different animals?

Apr 30, 2015/ Comments Off / in FAQ

The dose depends on the system or region being perfused.  For intravascular perfusion and after the blood is flushed with saline, infuse the equivalent of 30-40% volume/weight of BriteVu.  However, these are general guidelines that typically fit small animals less than 1,000 g.  See protocols for additional ideas. For example, perfuse 150-200 cc (or ml) of BriteVu into the vasculature of a 500 gram animal. Other systems, like the respiratory system for example, have very different volumes based on the species. …

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