BriteVu Perfused Alligator Kidney SEM

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Sep 13, 2015/ Off Comments / in Reptiles
BriteVu Perfused Alligator Kidney SEM

As part of a research project using scanning electron microscopy (SEM), BriteVu perfused alligator kidney was evaluated. Because of an equipment failure, the kidney tissue was not coated (which normally reduces electron charging). We decided to go ahead with the SEM anyways. The end result was that the tissues were overcharged (especially the BriteVu perfused vessels) creating bright spots. These bright spots actually highlight the location of the contrast agent within the tissues.  BriteVu is seen as the ‘bright spots’ in between the renal tubules and mark the location of the blood vessels.